Stable cell lines expressing recombinant antibodies are generated by experienced scientists working on antibody and cell line development services for decades. Stable cell lines developed with our proprietary recombinant antibody expression system with multiple selectable markers is very productive in expressing target proteins.
CHO suspension clonal stable cell lines or HEK 293 cells generated by electroporation using proprietary mammalian expression vector system with multiple selectable markers to simultaneously express two individual genes of interest.
Transfection into mammalian cells and screening for cell lines permanently expressing specific genes through their incorporation into the cellular genome. The standard method for screening clones is ELISA. Additional fee will be charged for Western blot or cell-based assay of your interest. Our fast and predictive screening techniques may significantly increase speed of stable cell line development while improving quality and uniformity.
Basic service of clonal stable cell line generation for antibody production includes:
a. Clone selection up to 24 clones;
b. Detailed characterization of the top clone during a 0.1 liter production run (viability, cell growth, and titer measurements);
c. Banking of 15 vials of the top clone (10 million cells per vial);
d. Backup vial storage for 6 months.
Deliverable:
a. 10 vials of the highest performing clone (10 million cells per vial);
b. All purified protein from 0.1 liter production run;
c. Stable cell line development study report.
Fast turnaround: 7-8 weeks.
Premium service of clonal stable cell line generation for antibody production includes basic service and the following:
a. Subcloning the top 3 clones for a second round of selection including screening of up to 72 clones;
b. Top three clones selected from the second round and characterized for viability, cell growth, and titer measurements during a 0.1 liter production run;
c. Improved expression levels, stability, and purity compared to basic service;
d. Detailed characterization of the final banked clone during a 1 liter production run starting from thaw of a frozen vial (viability, cell growth, and titer measurements);
e. Backup vial storage for 1 year.
Deliverable:
a. 10 vials of the highest performing clone (15 million cells per vial);
b. All purified protein from all production runs;
c. Stable cell line development study report.
Fast turnaround: 12-14 weeks.
Service of pool stable cell line generation for antibody production includes:
Deliverable:
a. 10 vials of the final pool (5 million cells per vial);
b. All purified protein from 0.1 liter production run;
c. Stable cell line development study report.
Fast turnaround: 6 weeks.